出厂物流调度问题及其启发式算法

针对出厂物流调度问题,建立了一类出厂物流问题的调度理论模型,证明了该问题的强NP-hard特性.基于对问题的特征分析,提出求解问题的近似算法,证明了算法的最坏性能比为3且为紧界,并通过数值仿真验证了该算法的有效性.结果表明,所提出算法对两台流水作业机器的完工时间有一定的优化.     

大学计算机基础课中基于问题式学习的探究实践

基于问题式学习（PBL）是一种以问题为导向的教学模式，能有效提高学生的学习兴趣，培养学生自主学习能力掌握解决问题的方法。罗群岛文中主要探索大学计算机基础课教学中基于问题式学习的教学设计策略与实施步骤，以期对大学计算机基础课改革有新的启示。     

间歇生产中过程调度的建模与分析

过程调度在间歇生产过程中起着非常重要的作用。针对过程调度的特点,我们建立了UIS中间存储策略下的间歇过程调度的混合整数规划(MILP)模型和时间Petri网模型,并分别采用Lindo和Hpsim1_1仿真工具对模型进行仿真实现。结果表明,对小规模问题,两种模型都能较好地解决,但前者约束多且描述复杂,而后者以图形化的工具描述单元间的逻辑关系,更适合调度系统的性能分析。     

挠性航天器大角度机动的振动抑制控制

建立了带变速控制力矩陀螺群的挠性航天器的动力学模型,应用误差四元数来描述姿态运动,将星体大角度姿态机动问题转化为误差四元数的调节问题.针对挠性航天器三轴同时姿态机动时挠性附件的振动抑制问题,提出了基于动态输出反馈控制的振动抑制方法,设计了仅利用姿态四元数而无需以角速度测量、挠性变形位移及速率测量作为反馈的动态控制规律.基于Lyapunov方法证明了所设计的动态控制器保证了姿态的渐近稳定和模态振动的衰减.基于Matlab/Simulink进行了仿真验证,结果表明了所提出的控制方法的可行性和有效性.     

Characterization of impurities in the bulk drug lisinopril by liquid chromatography/ion trap spectrometry

Two trace impurities in the bulk drug lisinopril were detected by means of high-performance liquid chromatography coupled with mass spectrometry （HPLC/MS） with a simple and sensitive method suitable for HPLC/MSn analysis. The fragmentation behavior of lisinopfil and the impurities was investigated, and two unknown impurities were elucidated as 2-（6-amino- l-（l-carboxyethylamino）- l-oxohexan-2-ylamino）-4-phenylbutanoic acid and 6-amino-2-（l-carboxy-3-phenylpropylamino）-hexanoic acid on the basis of the multi-stage mass spectrometry and exact mass evidence, The proposed structures of the two unknown impurities were further confirmed by nuclear magnetic resonance （NMR） experiments after preparative isolation.     

Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis

A new feather-degrading bacterium was isolated from a local feather waste site and identified as Bacillus subtilis based on morphological, physiochemical, and phylogenetic characteristics. Screening for mutants with elevated keratinolytic activity using N-methyl-N＇-nitro-N-nitrosoguanidine mutagenesis resulted in a mutant strain KD-N2 producing keratinolytic activity about 2.5 times that of the wild-type strain. The mutant strain produced inducible keratinase in different substrates of feathers, hair, wool and silk under submerged cultivation. Scanning electron microscopy studies showed the degradation of feathers, hair and silk by the keratinase. The optimal conditions for keratinase production include initial pH of 7.5, inoculum size of 2% （v/v）, age of inoculum of 16 h, and cultivation at 23 ℃. The maximum keratinolytic activity of KD-N2 was achieved after 30 h. Essential amino acids like threonine, valine, methionine as well as ammonia were produced when feathers were used as substrates. Strain KD-N2, therefore, shows great promise of finding potential applications in keratin hydrolysis and keratinase production.     

关于农村转移劳动力中存在的隐性失业问题的探讨

分析了我国农民工隐性失业问题的现状和原因，提出了促进我国劳动力市场协调发展的对策。     

求解同时取货和送货车辆路径问题的改进遗传算法

同时取货和送货车辆路径问题(VRP_SPD)是经典车辆路径问题(VRP)的一个扩展,在VRP_SPD中,顾客可能要求同时取货和送货服务。本文针对这类问题,提出一种以集成方式处理取货和送货操作的改进遗传算法,通过采用一种改进的边重组交叉算子,保证了算法在遗传进化中保留父代路径上边之间邻接关系的映射信息,从而改进了算法性能;并通过在遗传进化控制参数中应用自适应策略,提高了算法的稳健性。仿真分析表明,本文算法比现有算法能取得更好的优化结果,且具有很好的稳定性。     

二维对流-扩散方程反问题的遗传算法求解

给出了利用遗传算法求解二维定常对流一扩散方程参数反演的一种新方法,该方法把参数反演问题转化为优化问题求解。特别从多个初始点开始寻优,并借助交叉和变异算子来获得参数的全局最优解。数值模拟结果表明,该方法具有精度高且编程简单、易于计算机实现等特点。     

MGB probe assay for rapid detection of mtDNAl1778 mutation in the Chinese LHON patients by real-time PCR

Objective： Leber＇s hereditary optic neuropathy （LHON） is a maternally inherited degeneration of the optic nerve caused by point mutations of mitochondrial DNA （mtDNA）. Many unsolved questions regarding the penetrance and pathophysiological mechanism of LHON demand efficient and reliable mutation testing. This study aims to develop a minor groove binder （MGB） probe assay for rapid detection of mtDNA11778 mutation and heteroplasmy in Chinese LHON patients by real-time polymerase chain reaction （PCR）. Methods： Forty-eight patients suspected of having LHON and their maternal relatives underwent a molecular genetic evaluation, with 20 normal individuals as a control group at the same time. A real-time PCR involving two MGB probes was used to detect the mtDNA 1 1778 mutation and heteroplasmy. A linear standard curve was obtained by pUCmLHONG and pUCmLHONA clones. Results： All 48 LHON patients and their maternal relatives were positive for rntDNA11778 mutation in our assay, 27 heteroplasmic and 21 homoplasmic. Eighteen cases did not show an occurrence of the disease, while 9 developed the disease among the 27 heteroplasmic mutation cases. Eleven did not show an occurrence of the disease, while 10 cases developed the disease among 21 homoplasmic mutation cases. There was a significant difference in the incidence between the heteroplasmic and the homoplasmic mutation types. The time needed for running a real-time PCR assay was only 80 min. Conclusion： This real-time PCR assay is a rapid, reliable method for mtDNA mutation detection as well as heteroplasmy quantification. Detecting this ratio is very important for predicting phenotypic expression of unaffected carriers.